I am referring to KGP’s instruction where it says slot 4. I am afraid that’s not possible cause my 2 GPUs are covering that particular slot.
Where as my motherboard standard slot for my TB3-card is 3lot 3.
On my old X299 Prime Deluxe, I had mine in Slot 5, and I had to re-do the AML for it. Then I moved it, and again I re-did the AML again. So you can move it around, but to get the best out of it, your TB AML should be adapted to the slot it's placed in. It might work without the AML correctly compiled for the designated slot, but you must have at least an X4 slot for it. It works in an X8 or x16 slot as well.
On my old Asus X99 motherboard, I had it in Slot 5, and it worked there. There was nothing in the manual for a preferred slot. (I just checked the manual - I still have it, and there's nothing I found about a suggested slot for the TB Card.) But, if your motherboard manual suggests a slot, use it if you can. More importantly, is that what you must do, is in the BIOS, for the section that handles TB configuration is to properly identify the slot that the TB card is in. It really won't work until that matches.
So, one way it might work is to connect it (the TB Card) is to put it in the slot you want, and then go into the BIOS and check there for the available slot assignments (in the BIOS, under the Thunderbolt configuration, look there for PCIe assignments). If the slot you put the TB card in is available, you're good to go to try and see if you can get it to work.
And, you may have to move some stuff around until you can find a slot for the TB card, and your two graphics cards. Good luck.